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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT114 |
| Description | Anti-LPA receptor 5 Antibody (GPR92) |
| Alternate Names |
|
| Background Information | Lysophosphatidic acid receptor 5 (LPA receptor 5), also known as GPR92, is a G12/13 and Gq coupled receptor that mediates the signaling of LPA. Ligand binding to LPA receptor 5 may increase intracellular levels of cAMP and calcium, resulting in the activation of a wide range of effector molecules. Recent studies have demonstrated that LPA receptor 5 is abundantly expressed in human mast cells, and mediates the release of the macrophage inflammatory protein-1β (MIP-1β), indicating that LPA receptor 5 may play a role in innate and adaptive immunity. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect the LPA receptor 5 protein using this Anti-LPA receptor 5 (GPR92) validated for use in WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A representative lot detected LPA receptor 5 in non-transfected CHEM-1 cells and CHEM-1 cells transfected with LPAR5(GPR92) (Catalogue. No. HTS209C - Transfected Cell Line). Immunocychemistry Analysis: A 1:500 dilution from a representative lot detected LPAR5 in TF1 cells, non-transfected CHEM-1 cells and CHEM-1 cells transfected with LPAR5(GPR92) (Catalogue. No. HTS209C - Transfected Cell Line). NOTE: The protein used to make the transfected CHEM1 cell line is full length LPAR5. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the extracellular domain of human LPA receptor 5. |
| Epitope | Extracellular domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the extracellular domain of LPA receptor 5. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~37 kDa observed. The calculated molecular weight of this protein is 41 kDa, but can be observed at ~60 kDa due to high glycosylation. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in K562 cell lysate. Western Blot Analysis: 0.2 µg/mL of this antibody detected LPA receptor 5 on 10 µg of K562 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |