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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B, Ca | ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT10 |
| Description | Anti-CKAP5 Antibody |
| Alternate Names |
|
| Background Information | Cytoskeleton-associated protein 5 (CKAP5) is a member of the TOG/XMAP215 family containing heat repeats and plays a role in kinetochore mircotubule protection from depolymerization by MCAK. CKAP5 is the homolog of the Xenopus protein, XMAP25 (also known as chTog), which plays an essential role in centrosomal assembly. CKAP5 is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Studies show CKAP5 interacts with TACC1, TACC3, and tubulin leading to the hypothesis that it functions in spindle microtuble organization. Hepatomas and colonic tumors have shown overexpression of CKAP5. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CKAP5 Antibody is an antibody against CKAP5 for use in IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:250 dilution from a previous lot detected CKAP5 in RPE1 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human CKAP5. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, and Canine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Plays a major role in organizing spindle poles. SUBUNIT STRUCTURE: Interacts with TACC1. TISSUE SPECIFICITY: Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. SEQUENCE SIMILARITIES: Belongs to the TOG/XMAP215 family. Contains 10 HEAT repeats. SEQUENCE CAUTION: The sequence AAH17856.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH70136.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAI11044.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAI20871.1 differs from that shown. Reason: Frameshift at position 18. The sequence BAA07892.2 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | 226 kDa calculated. Three isoforms are produced by alternative splicing with molecular weights of 225, 218, and 226 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CKAP5 in A431 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |