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Millipore Anti-Mtor Antibody Detects Level Of Phospho-Mtor (Ser2159) & Has Been Published & Validated For Use In Western Blotting, Peptide Inhibition Assay, Dot Blot. - Mill (Additional S&H or Hazmat Fees May Apply)
FK506-binding protein 12-rapamycin complex-associated protein 1
FKBP12-rapamycin complex-associated protein
Mammalian target of rapamycin
mTOR
Mechanistic target of rapamycin
Rapamycin and FKBP12 target 1
Rapamycin target protein 1
Background Information
The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.
Product Information
Format
Affinity Purified
Control
Unmodified and modified mTOR peptides
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot.
Key Applications
Western Blotting
Peptide Inhibition Assay
Dot Blot
Application Notes
Western Blot Analysis: A representative lot detected phospho-mTOR (Ser2159) in HEK293 cell lysate. Image courtesy from the laboratory of Dr. Diane Fingar, University of Michigan Medical School.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Ser2159.
Epitope
Phosphorylated at Ser2159
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes mTOR phosphorylated at Ser2159.
Species Reactivity
Human
Mouse
Rat
Sheep
Zebrafish
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Mouse, Rat, Zebra Fish, and Sheep based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_004949
Gene Symbol
MTOR
FRAP
FRAP1
FRAP2
RAFT1
RAPT1
Purification Method
Affinity Purfied
UniProt Number
P42345
Molecular Weight
290 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Dot Blot in unmodified and modified mTOR peptides.
Dot Blot Specificity Analysis: Unmodified and modified mTOR peptides (see Table) were probed with Anti-phospho-mTOR (Ser2159) (1:1,000 dilution).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
