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Millipore Anti-Colipase Antibody | Abs543 Is An Antibody Against Colipase Antibody For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
The protein Colipase is a co-enzyme needed for maximal enzyme activity of pancreatic lipase. It is a secreted protein produced by the pancreas that is originally secreted as an inactive proform, procolipase. Procolipase gets activated by cleavage by intestinal trypsin in the lumen of the small intestine. Once activated, Colipases function is to help overcome the inhibitory effect of bile salts on the lipase catalyzed hydrolysis of long-chain triglycerides that needs to occur within the duodenum. Colipase happens to have key hydrophobic amino acids that allow the complex of lipase and Colipase to adsorb to the lipid micelles, and this adsorption is essential for their digestion. Interestingly too, the procolipase propeptide also gives rise to the pentapeptide intestinal hormone Enterostatin, a small pentapeptide. Enterostatin acts to reduce food intake, in particular fat intake, when given to animals via injection or in to the brain.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Colipase Antibody | ABS543 is an antibody against Colipase Antibody for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Colipase in purified protein (DSilva, S., et al. (2007). Journal of Lipid Research. 48:2478-2484). Western Blotting Analysis: A representative lot detected Colipase in mouse pancreas (Miller, R., et al. (2009). AM J Physiol Endocrinol Metab. 297:E856-E865). Western Blotting Analysis: A representative lot detected Colipase in HEK293T cells transiently transfected to express Arg92 and Cys92 colipase (Xiao, X., et al. (2013). Journal of Lipid Research. 54:514-521).
Biological Information
Immunogen
Recombinant protein corresponding to human Colipase.
Epitope
Unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001823
Gene Symbol
CLPS
Purification Method
Unpurified
UniProt Number
P04118
Molecular Weight
~12 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse pancreas tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Colipase in mouse pancreas tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
