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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS449 |
| Description | Anti-UNG1 Antibody |
| Alternate Names |
|
| Background Information | Uracil-DNA glycosylase 1 (UNG1), also known as UDG1, DGU or UNG15, hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, and as a result it releases free uracil. UNG1 excises uracil residues from the DNA. This event could be a result of faulty incorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Uniport describes two isoforms. The fist isoform is widely expressed with the highest expression in skeletal muscle, heart and testicles. The second isoform has the highest expression levels in tissues containing proliferating cells. Recent studies have shown that defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). HIGM5 is a rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-UNG1 Antibody is a highly specific rabbit polyclonal antibody, that targets Uracil-DNA glycosylase 1 & has been tested in western blotting & IHC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 2 µg/mL from a representative lot detected UNG1 in human heart tissue. Immunofluorescence Analysis: 20 µg/mL from a representative lot detected UNG1 in human heart tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminus of human UNG1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the C-terminus of UNG1. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Antigen Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~37 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~34 kDa and ~35 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in C2C12 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected UNG1 in 15 µg of C2C12 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |