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Millipore Detect Lipocalin Using This Rabbit Polyclonal Antibody, Anti-Lcn13 Antibody Validated For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Epididymal-specific lipocalin-13 (LCN13) is also called Odorant-binding protein 2a (Obp2a). LCN13 is a secreted protein that is thought to act as a retinoid carrier protein within the epididymis, and have a role in male fertility. LCN13 is expressed in the epididymis.
Product Information
Format
Serum
Control
Mouse skin tissue lysate
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Lipocalin using this rabbit polyclonal antibody, Anti-LCN13 Antibody validated for use in western blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LCN13 in mouse lung tissue lysate.
Western Blotting Analysis: A representative lot from an independent laboratory detected LCN13 in liver, muscle, and pancreas tissues, but not in white adipose tissue, from LCN13 transgenic male mice (Cho, K. W., et al. (2011). Mol Cell Biol. 31(3):450-7.).
Western Blotting Analysis: A representative lot from an independent laboratory detected LCN13 in transfected mouse primary hepotcytes cell lysates (Sheng, L, et al. (2011). J Biol Chem. 286(44):38128-38135.).
Western Blotting Analysis: A representative lot detected LCN13 in mouse adipocyte cell lysates and in certain mouse organ tissues lysates (Zhou, Y. and Rui, L., (2013). Vitam Horm. 91:369-383.).
Biological Information
Immunogen
Recombinant protein corresponding to mouse LCN13.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody detects two observed isoforms of LCN13.
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_705786
Gene Symbol
Obp2a
Lcn13
Purification Method
Unpurified
UniProt Number
Q8K1H9
Molecular Weight
~20 kDa observed. Two observed isoforms at ~20 kDa and ~28 kDa are likely due to proteolytic cleavage of a common LCN13 precursor (Cho, K. W., et al. (2011). Mol Cell Biol. 31(3):450-7.). Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse skin tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LCN13 in 10 µg of LCN13 mouse skin tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
