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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, IF, ICC, ChIP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1023 |
| Description | Anti-Angiomotin Antibody |
| Alternate Names |
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| Background Information | Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Angiomotin Antibody | ABS1023 is an antibody against Angiomotin Antibody for use in Western Blotting, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected phospho Angiomotin (Ser175) in HEK293 transfected with AMOT cell lysate (Dr. Joseph Kissil, Scripps Research Institute, Florida). Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells expressing equal amount of HA-Merlin and Myc-Rich1, untreated (−) or Doxycyline induced (+) RT4-67 cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540). Western Blotting Analysis: A representative lot detected Angiomotin in Flag-IP and total cell lysate from HEK293 cells transfected with Flag-tagged wild-type (WT) or patient-derived mutant Merlin alleles, HEK293, ERK activation in Nf2−/− SC4 cells or Merlin-reconstituted SC4 cells (Nf2−/−+retroNf2) (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540). Western Blotting Analysis: A representative lot detected Angiomotin in HEK293, MEFs treated with Adeno (−) or Adeno-Cre (+) virus (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12). Immunofluorescence Analysis: A representative lot detected Angiomotin in epithelial cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540). Immunofluorescence Analysis: A representative lot detected Angiomotin in liver from Amot KO mice (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12). Immunocytochemistry Analysis: A representative lot detected Angiomotin in SC4 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12). Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human Angiomotin. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody detects p80 and p130 forms of AMOT |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | 150/80 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 transfected with LATS cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Angiomotin in 10 µg of HEK293 transfected with LATS cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |