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Millipore Detect S100 Using This Rabbit Polyclonal Antibody, Anti-S100b Antibody Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect S100 using this rabbit polyclonal antibody, Anti-S100B Antibody validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected S100B in human thalamus and human cerebellum sections.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human S100B near the N-Terminus.
Epitope
Near N-Terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Predicted to react with Mouse, Rat, Bovine, and Rhesus Macaque based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_006263
Gene Symbol
S100B
Purification Method
Affinity Purfied
UniProt Number
P04271
Molecular Weight
~10 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A375 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected S100B in 10 µg of A375 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
