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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P), ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN410 |
| Description | Anti-ZNF423 Antibody |
| Alternate Names |
|
| Background Information | ZNF423, also known as Zinc finger protein 423, Olf1/EBF-associated zinc finger protein, hOAZ, or Smad and Olf-interacting zinc finger protein, and encoded by the gene ZNF423/KIAA0760/NPHP14/OAZ, is a transcription factor that can both act as an activator or a repressor depending on the context. ZNF423 plays a central role in BMP signaling and olfactory neurogenesis. ZNF423 acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation. ZNF423 is involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons and it controls the proliferation and differentiation of neural precursors in cerebellar vermis formation. ZNF423 is expressed in many organs including the brain, lung, skeletal muscle and heart in both fetal and adult states. Defects in ZNF423 cause the genetic kidney disease nephronophthisis and the neurological disorder called Joubert syndrome. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ZNF423 Antibody is an antibody against ZNF423 for use in western blotting, IHC (Paraffin) & ICC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:500 and a 1:50 dilution from a representative lot detected ZNF423 in human and rat cerebellum tissues, respectively. Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected ZNF423 in human thalamus and hippocampus tissues. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZNF423 in NIH/3T3 cells. DyLight® is a registered trademark of Thermo Fisher Scientific. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a region near the C-terminus of human ZNF423. (Note: The immunogen contains 93% sequence homology with bovine.) |
| Epitope | Near C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse P7 brain tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected ZNF423 in 10 µg of mouse P7 brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |