Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN37 |
| Description | Anti-Homer1 Antibody |
| Alternate Names |
|
| Background Information | Homer protein homolog 1 (UniProt: Q86YM7; also known as Homer-1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID: 9456) in human. Homer1, 2, and 3 constitute a family of postsynaptic scaffold proteins that play a central role in calcium signaling. Homer1 is a widely studied member of this family, comprising both synaptic structure and mediating postsynaptic signaling transduction. It is a tetrameric protein that binds and cross-links cytoplasmic regions of various proteins. It contains a WH1 domain (aa 1-110) that interacts with the PPXXF motif in GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1, and SHANK3. It also contains a C-terminal coiled-coil region (aa 181-352) region that mediate self-multimerization. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, Homer1 helps coupling of surface receptors to intracellular calcium release. Three isoforms of Homer1 have been described that are produced by alternative splicing. Isoform 1 is reported to regulate the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3 may also be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Homer1 can negatively regulate T cell activation by inhibiting the calcineurin-NFAT pathway. It acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by protein phosphatase 3 catalytic subunit (PPP3CA). (Ref.: Luo, P., et al. (2012). Neurochem. Int. 61(5); 731-738; Huang, GN., et al. (2008). Science. 319(5862); 476-481). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Homer1, Cat. No. ABN37, is a rabbit polyclonal antibody that detects Homer 1 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Homer1 in human brain and mouse brain tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the internal region of human Homer1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Homer1. It targets an epitope within 12 amino acids from the internal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Canine, Feline, Monkey, Horse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. |
| Gene Symbol |
|
| Purification Method | Affinity purfied. |
| UniProt Number |
|
| Molecular Weight | ~47 kDa observed; 40.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Mouse brain tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Homer protein homolog 1 in Mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |