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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M, Chp | WB, IP, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN27 |
| Replaces | 06-986 |
| Description | Anti-GRIP-1 Antibody, CT |
| Alternate Names |
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| Background Information | Glutamate receptor interacting protein-1 (GRIP-1) is a multi-PDZ domain scaffold protein and a member of the steroid receptor coactivator (SRC) family of proteins. GRIP-1 interacts and maneuvers kinesin heavy chains to dendrites by acting as the power source for AMPA receptors. During initial stages of embryonic development, GRIP-1 is essential for typical cell-matrix interactions. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect GRIP-1 using this Anti-GRIP-1 Antibody, C-terminus validated for use in WB, IP, IH(P). |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated GRIP-1 from rat brain cytosol tissue lysate. Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected GRIP-1 in rat brain cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminus of rat GRIP-1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes GRIP-1 at the C-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with rat. Predicted to react with human, mouse, and chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Binds to the liprin-alpha SVD2 family of proteins to mediate synaptic targeting of AMPA receptors; may play a role in presynaptic and postsynaptic maturation [RGD]. |
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons. SUBUNIT STRUCTURE: Interacts with EFNB1, EPHA7, EPHB2, EFNB3, KIF5A, KIF5C, KIF5B and the C-terminal tail of PRLHR. Forms a ternary complex with GRIA2 and CSPG4 (By similarity). Can form homomultimers or heteromultimers with GRIP2. Interacts with GRIA2, GRIA3, GRIPAP1/GRASP1, PPFIA1, PPFIA4, FRAS1, PLCD4, PTPRF and liprins-alpha. Interacts with SLC30A9 (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Endomembrane system; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Endoplasmic reticulum membrane; Peripheral membrane protein (Potential). Note: Membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities. TISSUE SPECIFICTY: Expressed in brain, testis and retina. In brain highly expressed in the olfactory bulb, cortex and hyppocampus and lower level in thalamus, cerebellum and spinal cord. In brain it is found in the perikaryon, dendrites, dendritic shafts, dendritic spines and, excitatory and inhibitory synapses of neurons. In retina, it is most abundant in the plexiform layers than in perikarya. DEVELOPMENTAL STAGE: Detected in early embryonic stage as early as E15, gradually increased throughout early development, peaked at approximately between postnatal days P6 and P8, then slightly decreased and remained relatively stable in the adult. DOMAIN: PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with PRLHR (By similarity). PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1. SEQUENCE SIMILARITIES: Contains 7 PDZ (DHR) domains. |
| Molecular Weight | ~121 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in rat brain cytosol tissue lysate. Western Blot Analysis: 0.05 µg/mL of this antibody detected GRIP-1 on 10 µg of rat brain cytosol tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |