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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IP, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN26 |
| Replaces | 06-573 |
| Description | Anti-iNOS/NOS II Antibody, NT |
| Alternate Names |
|
| Background Information | Nitric oxide synthase, inducible (UniProt: P29477; also known as EC:1.14.13.39, Inducible NO synthase, Inducible NOS, iNOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2) is encoded by the Nos2 (also known as Inosl) gene (Gene ID: 18126) in murine species. Nitric oxide synthases (nNOS, iNOS, eNOS) are single polypeptides that differ in their rates of Nitric oxide ( NO) synthesis, Ca2+- dependence, cytochrome c reduction, and NADPH utilization. For their catalytic activities NOS isoforms require three distinct domains: (a) a reductase domain, (b) a calmodulin-binding domain, and (c) an oxygenase domain. The reductase domain contains the FAD and FMN moieties. The oxygenase domain, which contains the binding sites for heme, tetrahydrobiopterin, and arginine, catalyzes the conversion of L-arginine to citrulline and NO. The maximal rate of NO synthesis is established by the intrinsic maximum ability of the reductase domain to deliver electrons to the heme domain. The iNOS is expressed in a variety of cells and is localized mainly in the cytoplasm. It binds tightly to calmodulin at very low Ca2+ levels, hence the enzyme activity is unaffected by changes in intracellular Ca2+ levels. The complete Ca2+-independence of inducible NOS requires interactions of calmodulin with both the calmodulin-binding motif and the flavoprotein domain. This property is important in assuring a long-lasting release of NO. The iNOS is capable of producing large amounts of NO when induced by mediators such as TNF- , IL-1, IL-2, IL-6, and -IFN. The amount of NO released by iNOS falls in the nanomolar range. The iNOS-induced release of NO plays an important role in the macrophage-mediated response to infections, where NO acts as an effector molecule to kill the invading organism. This toxic effect is achieved via the production of highly reactive nitrogen and oxygen intermediates. The induction of iNOS has been implicated in increased blood flow to the tumor site resulting in tumor growth. The cytotoxic effects of NO have also been reported against cancer cells. Over-expression of iNOS has been implicated in a number of pathologies including septic shock where excessive generation of NO, in response to infection, leads to a serious drop in blood pressure and subsequent dysfunction of multiple organs. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-iNOS/NOS II, NT, Cat. No. ABN26, is a rabbit polyclonal antibody that detects inducible nitric oxide synthase (iNOS) and is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µg from a representative lot detected iNOS/NOS II, NT in RAW 234.7 treated with INFgamma/LPS. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected iNOS/NOS II, NT in human lung cancer and human colon cancer tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to the first 130 amino acids from the N-terminal region of murine inducible nitric oxide synthase (iNOS). |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects inducible nitric oxide synthase (iNOS). It targets an epitope within the N-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. CATALYTIC ACTIVITY: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+. COFACTOR: Heme group. Binds 1 FAD. Binds 1 FMN. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. ENZYME REGULATION: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. SUBUNIT STRUCTURE: Homodimer. Binds SLC9A3R1 (By similarity). TISSUE SPECIFICTY: Macrophages. INDUCTION: By treatment with endotoxins or cytokines. SEQUENCE SIMILARITIES: Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. |
| Molecular Weight | ~130 kDa observed; 135.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in RAW 234.7 treated with INFgamma/LPS cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected iNOS/NOS II, NT in RAW 234.7 treated with INFgamma/LPS cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |