Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1389 |
| Description | Anti-MARCO |
| Alternate Names |
|
| Background Information | Macrophage receptor MARCO (UniProt: Q9UEW3; also known as macrophage receptor with collagenous structure; Scavenger receptor class A member 2) is encoded by the MARCO (also known as SCARA2) gene (Gene ID: 8685) in human. MARCO is strongly expressed in macrophages of several tissues in humans with sepsis. It is a scavenger receptor that is readily upregulated following bacterial infection. Hence, it forms a back-up system through which the organism can quickly arm more cells with highly efficient phagocytosing receptors. MARCO is a single-pass type II membrane protein that binds both Gram-positive and Gram-negative bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. The bacteria-binding region is localized immediately proximal to the cysteine-rich region of domain V. Human and mouse MARCO exhibit about 68% sequence identity. However, the human MARCO subunit chain differs from the mouse chain in that it does not have any cysteine residues outside the cysteine-rich domain V, whereas the mouse chain has one cysteine residue within the intracellular domain I and two in the extracellular spacer domain III. (Ref.: Elomaa, O., et al. (1998). J. Biol. Chem. 273(8): 4350-4538). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MARCO, Cat. No. ABN1389, is a highly specific rabbit polyclonal antibody that targets Macrophage receptor MARCO and has been tested in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected MARCO in 10 µg of A549 cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-cojugated peptide corresponding to 12 amino acids from the C-terminal region of human MARCO. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects macrophage receptor MARCO in RAW264.7 cells. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~60 kDa observed; 52.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in RAW264.7 cells. Western Blotting Analysis (WB): A 1:5000 Dilution of this antibody detected Macrophage receptor MARCO in 10 µg RAW264.7 cells . |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |