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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1383 |
| Description | Anti-phospho-TrkA (Tyr794) Antibody |
| Alternate Names |
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| Background Information | High affinity nerve growth factor receptor (EC 2.7.10.1; UniProt P35739; also known as Neurotrophic tyrosine kinase receptor type 1, p140-TrkA, Slow nerve growth factor receptor, Trk-A, TrkA neurotrophin receptor, TrkA proto-oncogene receptor, Tropomyosin-related kinase A) is encoded by the Ntrk1 gene (also known as Trk, Trka) in rat (Gene ID 59109). Neurotrophins mediate a wide range of neuronal functions, including survival, differentiation, growth, and death, via two classes of transmembrane receptors, the Trk receptor tyrosine kinase and the p75 neurotrophin receptor. While nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and NT-4 all bind the p75 receptor, TrkA is selective for NGF, TrkB is selective for BDNF and NT-4, and TrkC is selective for NT-3. TrkA and TrkB receptors can also be activated by ligands of the G-protein-coupled receptor (GPCRs), including the nucleoside adenosine or adenosine agonists, such as CGS 21680 and the pituitary adenylate cyclase-activating polypeptide (PACAP). In addition to transactivation via GPCRs, Glucocorticoids (GCs) are also reported to promote TrkA/B/C activation due to signaling cross-talk between Trk and GC receptor (GR). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-TrkA (Tyr794) using this Anti-phospho-TrkA (Tyr794) Antibody validated for use in Western Blotting and Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected NGF-stimulated Tyr794 phosphorylation of exogenously expressed human TrkA in PC12 cells (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University). Immunocytochemistry Analysis: A representative lot detected PACAP-induced TrkA Tyr794 phosphorylation in cultured rat basal forebrain neurons (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University). Western Blotting Analysis: A representative lot detected NGF-stimulated Tyr794 phosphorylation of exogenously expressed human TrkA in PC12 cells (Rajagopal, R., et al. (2004). 24(30):6650-6658). Western Blotting Analysis: A representative lot detected NGF-stimulated TrkA Tyr794 phosphorylation in cultured rat embryonic E18 septal neurons (Jeanneteau, F., et al. (2008). Proc Natl Acad Sci USA. 105(12):4862-4867). Western Blotting Analysis: A representative lot detected NGF-dependent TrkA Tyr794 phosphorylation in cultured DRG neurons from E16.5 rat embryos (Arevalo, J.C., et al. (2006). Neuron. 50(4):549-559). Immunocytochemistry Analysis: A representative lot detected PACAP38-induced TrkA Tyr794 phosphorylation in cultured rat basal forebrain neurons, as well as CGS 21680- and NGF-induced Tyr794 phosphorylation of exogenously expfressed human TrkA in serum-starved PC12 cultures (Rajagopal, R., et al. (2004). 24(30):6650-6658). Immunocytochemistry Analysis: A representative lot detected NGF-stimulated TrkA Tyr794 phosphorylation in cultured DRG neurons from E16.5 rat embryos (Arevalo, J.C., et al. (2006). Neuron. 50(4):549-559). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to rat TrkA sequence near the C-terminus with phosphorylated Tyr794. |
| Epitope | Near C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody selectively detects NGF-induced pTyr794 phosphorylation of TrkA in cultured primary embryonic rat septal neurons, but not BDNF-induced pTyr816 phosphorylation of TrkB in rat hippocampal neurons (PMID 18347336). |
| Species Reactivity |
|
| Species Reactivity Note | Rat, Human. Predicted to react with Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~140 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NGF treated human TrkA-transfected PC12 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected phospho-TrkA (Tyr794) in 10 µg of NGF treated human TrkA-transfected PC12 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |