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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1382 |
| Description | Anti-DGL-α Antibody |
| Alternate Names |
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| Background Information | The protein DGL-alpha is a lipase enzyme that catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG) endocannabinoid in tissues. Endocannabinoids are a group of lipid-like molecules that are neuromodulatory in nature and are involved in the control of many physiological functions including appetite, pain sensation, mood, and memory. They also mediate the psychoactive effects of cannabis (marijuana). Endocannabinoids (ECs) act as retrograde messengers that enable postsynaptic cells to regulate the strength of their synaptic inputs. DGL-alpha is also an excellent marker for cerebellum as DGL-alpha is highly expressed in cerebellum and necessary for the regulation of synaptic retrograde transmission in the cerebellum, hippocampus and striatum, as well as other regions including the auditory brainstem. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-DGL-α Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of DGL-α. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DGL-α in human cerebellum and mouse cerebellum tissue. Immunohistochemistry Analysis: A representative lot detected DGL-α in mouse brain tissue (Berghuis, P., et al. (2007). Science. 316:1212-1216). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the cytoplasmic domain of human DGL-α. |
| Epitope | Cytoplasmic domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse cerebellum tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DGL-α in 10 µg of mouse cerebellum tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |