Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1370 |
| Description | Anti-MTNR1B |
| Alternate Names |
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| Background Information | Melatonin receptor type 1B (UniProt: P49286; also known as Mel-1B-R; Mel1b receptor) is encoded by the gene MTNR1B gene (Gene ID 4544) in human. Mel-1B-R is a high affinity receptor for melatonin that belongs to the G-protein coupled receptor 1 family. Mel-1B mediates the reproductive and circadian actions of melatonin. Melatonin is shown to be involved in body mass regulation in mammals and this effect is suggest to be mediated by a direct action of melatonin on Mel-1B receptors on brown adipocytes. Melatonin also acts as a chemopreventive agent and its levels inversely correlate with the risk of developing cancer. In this regard melatonin induces p38-dependent phosphorylation of both p53 and histone H2AX. Activation of the p53-dependent DNA damage response by melatonin is mediated by both Mel-1A and Mel-1B receptors. The absence of either receptor impairs melatonins ability to reduce both cell proliferation and clonogenic potential of cancer cells. (Brydon, L. et al. (2001). Endocrinology. 142(10):4264-4271; Santoro, R., et al. (2013). Carcinogenesis. 34(5):1051-1061). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-MTNR1B, Cat. No. ABN1370 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Melatonin receptor type 1B. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MTNR1B in human retina and human melanoma tissue. |
| Biological Information | |
|---|---|
| Immunogen | A KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal end of human melatonin receptor type 1B. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects melatonin receptor type 1B in human hippocampus membrane. |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~38 kDa observed; 40.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human hippocampus membrane. Western Blotting Analysis: 2 mg/mL of this antibody detected MTNR1B in 10 mg of human hippocampus membrane. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |