Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1365 |
| Description | Anti-OSTM1 |
| Alternate Names |
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| Background Information | Osteopetrosis-associated transmembrane protein 1 (UniProt: Q86WC4; also known as Chloride channel 7 beta subunit) is encoded by the OSTM1 (also known as GL) gene (Gene ID: 28962) in human. Osteopetrosis-associated transmembrane protein 1 is a single-pass type I membrane protein that is required for osteoclast and melanocyte maturation and function. It is synthesized with a signal peptide of 31 amino acids, which is cleaved to produce the mature protein. Loss of OSTM1 leads to the most severe form of osteoporosis in mice and human. Although its role in bone chemistry is well established, several studies have shown its expression in neurons, microglia, and astrocytes. OSTM1-deficiecy in the central nervous system leads to gliosis with increased astrocyte cell population and microglia activation along with retinal degeneration at 2 to 3 weeks of age in mice. (Ref.: Ref.: Heraud, C., et al. 2014. J. Biol. Chem. 289(20); 13912-25). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-OSTM1, Cat. No. ABN1365, is a highly specific rabbit polyclonal antibody that targets Osteopetrosis-associated transmembrane protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OSTM1 in human pancreas and human liver tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminus region of human Osteopetrosis-associated transmembrane protein 1 (OSTM1). |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Osteopetrosis-associated transmembrane protein 1 in human cells. It targets an epitope within 18 amino acids from the C-terminus region. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Baboon, Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~37 kDa observed; 37.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human liver tissue lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected OSTM1 in 20 µg of human liver tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |