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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | WB, IF | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1362 |
| Description | Anti-RBPMS Antibody |
| Alternate Names |
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| Background Information | RNA-binding protein with multiple splicing (UniProt Q93062; also known as Heart and RRM expressed sequence, Hermes, RBP-MS) is encoded by the RBPMS (also known as HERMES) gene (Gene ID 11030) in human. RBPMS is a member of the RRM (RNA recognition motif) family of RNA-binding proteins. Transcriptome-wide study using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in HEK293 cells reveals both exonic mature and intronic pre-mRNA-binding sites, in agreement with the nuclear and cytoplasmic localization of the RRM family proteins. RBPMS is found to be exclusively expressed in retinal ganglion cells (RGCs) and displaced RGCs (dRGCs). RBPMS expression can therefore be utitized as a RGC marker, and the RBPMS gene promoter could be utilized for driving RGC-specific transgene expression. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 1% BSA and 0.09 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-RBPMS Antibody is an antibody against RBPMS for use in Western Blotting, Immunofluorescence. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected endogenous RBPMS in mouse and rat retina extracts, as well as exogenously expressed human RBPMS in transfected HEK293T cells (Rodriguez, A.R., et al. (2014). J. Comp. Neurol. 522(6):1411-1443). Immunofluorescence Analysis: A representative lot detected RBPMS immunoreactivity primarily associated with cell bodies located in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry using whole-mounted retinas (Rodriguez, A.R., et al. (2014). J. Comp. Neurol. 522(6):1411-1443). Immunofluorescence Analysis: An 1:5,000 dilution of a representative lot detected RBPMS immunoreactivity among cells in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry (Courtesy of Dr. Nicholas Brecha, David Geffen School of Medicine at UCLA). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence from the N-terminal region of human RBPMS. |
| Epitope | N-terminal region. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with all five spliced isoforms (UniProt Q93062-1 through Q93062-5). |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~25 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse embryonic stem cell (mESC) lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected RBPMS in 10 µg of mouse embryonic stem cell (mESC) lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |