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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, Rhesus Macaque | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN117 |
| Description | Anti-PDE4D3 Antibody |
| Alternate Names |
|
| Background Information | Phosphodiesterase 4D3 (PDE4D3) is a member of the PDE cyclic nucleotides. PDE4D3 is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D3 is a direct target of the MSH/cAMP/MITF pathway and may play a role in melanocyte differentiation. PDE4D3 is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D3 may be associated with analgesic and anti-inflammatory effects. PDE4D3 has also been linked to the risk of stroke, and in particular to polygenic stroke. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PDE4D3 Antibody is validated for use in WB for the detection of PDE4D3. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of human PDE4D3. |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes PDE4D3 at the N-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse and Rat. Predicted to react with Rhesus Monkey based on 100% sequence homology. Predicted to react with Human based on immunogen design. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes one of four mammalian counterparts to the fruit fly dunce gene. The encoded protein has 3,5-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. CATALYTIC ACTIVITY: Adenosine 3,5-cyclic phosphate + H2O = adenosine 5-phosphate. COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by rolipram. Activated by phosphatidic acid. PATHWAY: Purine metabolism; 3,5-cyclic AMP degradation; AMP from 3,5-cyclic AMP: step 1/1. SUBUNIT STRUCTURE: Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Cytoplasm › cytoskeleton (By similarity). Cytoplasm › cytoskeleton › centrosome (By similarity). Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). TISSUE SPECIFICTY: Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. PTM: Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. INVOLVEMENT IN DISEASE: Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. Ref.16 states that association with stroke has to be considered with caution. SEQUENCE SIMILARITIES: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. |
| Molecular Weight | ~95 kDa observed. UniProt describes 12 isoforms produced by alternative splicing. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain membrane tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PDE4D3 on 10 µg of mouse brain membrane tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |