Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1000 |
| Description | Anti-MAGL Antibody |
| Alternate Names |
|
| Background Information | Monoglyceride lipase (MGL), or alternatively HU-K5, Lysophospholipase homolog, Lysophospholipase-like, or Monoacylglycerol lipase (MAGL) is a protein encoded by the MGLL gene in humans and is very important in lipid metabolism. Monoglyceride lipase is the enzyme that converts monoacylglycerides (key building blocks of lipids) into free fatty acid chains and glycerol. Also, Monoglyceride Lipase hydrolyzes endocannabinoids which ultimately can regulate nociperception and the perception of pain, so the enzyme is being studied in pain mediation therapies. Monoglyceride Lipase is expressed in many tissues including fat, lung, liver, brain and heart. In disease, Monoglyceride Lipase is being studied most intensely in cancer research. In some cancers it appears to be play a suppressive role in regulating AKT mediated signaling, but in others, since the enzyme regulates the levels of fatty acids that can serve as intra and intercellular signaling molecules, Monoglyceride lipase activity seems to promote cancer cell migration, invasion and growth. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MAGL Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of MAGL. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MAGL in human cerebral cortex tissue. Immunohistochemistry Analysis: A representative lot detected MAGL in human hippocampus tissue (Mulder, J., et al. (2011). Brain. 134:1041-1060). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse MAGL. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~ 31/33 kDa observed. This protein can be alternatively spliced, so western blots may show a doublet. Evidence for alternative splicing of MAGL, can run as doublet, ~31 and ~33 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected MAGL in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |