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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Rhesus Macaque | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABF29 |
| Description | Anti-NLRP6 (NALP6) Antibody |
| Alternate Names |
|
| Background Information | NRLP6 (NALP6) (also known as PYRIN-containing Apaf-1-like protein 5 or PYPAF5) belongs to a family of proteins that play a critical role in apoptosis and in inflammatory signaling. Its expression is highly restricted to granulocytes and T-cells. Co-expression of NALP6 with ASC results in synergistic activation of caspase-1-dependent cytokine processing, subsequent NF-kB activation, and downstream recruitment of PYPAF5 to punctate structures in the cytoplasm. Overlapping genes in NALP6/PYPAF5 locus encode two V2-type vasopressin isoreceptors: angiotensin-vasopressin receptor (AVR) and non-AVR. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NLRP6 (NALP6) Antibody detects level of NLRP6 (NALP6) & has been published & validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NLRP6 (NALP6) in epithelial tissue of proximal and distal tubules of human kidney, and in human bone marrow tissue. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to NACHT domain of human NLRP6 (NALP6). |
| Epitope | NACHT domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the NACHT domain of NLRP6 (NALP6). |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Rat. Predicted to react with Rhesus Macaque based on 100% sequence homology. Other homologies: Mouse (93% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | NALPs are cytoplasmic proteins that form a subfamily within the larger CATERPILLER protein family. Most short NALPs, such as NALP6, have an N-terminal pyrin (MEFV; MIM 608107) domain (PYD), followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. The long NALP, NALP1 (MIM 606636), also has a C-terminal extension containing a function to find domain (FIIND) and a caspase recruitment domain (CARD). NALPs are implicated in the activation of proinflammatory caspases (e.g., CASP1; MIM 147678) via their involvement in multiprotein complexes called inflammasomes. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B. SUBUNIT STRUCTURE: Binds to ASC with its DAPIN domain. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highly expressed in granulocytes. Detected at much lower levels in T-cells. |
| Molecular Weight | ~99 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in rat basophilic leukemia (RBL) cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected NLRP6 (NALP6) in 10 µg of rat basophilic leukemia (RBL) cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |