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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABF184 |
| Description | Anti-IL-21 Antibody |
| Alternate Names |
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| Background Information | Interleukin-21 (UniProt Q9HBE4; also known as IL-21, Za11) is encoded by the IL21 (also known as CVID11) gene (Gene ID 59067) in human. IL-21 is a type I four-alpha-helical bundle cytokine primarily produced by CD4+ T cells and NKT cells. The heterodimeric receptor IL-21R is dominantly expressed by B cells and shares the common cytokine receptor gamma chain (γc) with receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. Studies indicate that IL-21 potently regulates both adaptive and innate immune responses and possesses widely pleiotropic functions. IL-21 has also been linked to autoimmune disorders, allergies, inflammatory diseases, as well as the pathogenesis of cancer. Human IL-21 is initially translated with an N-terminal 22 aa signal peptide sequence, which is later removed to produce the 133 aa mature IL-21. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit Polyclonal Anti-IL-21 Antibody, Cat. No. ABF184 is validated for use in Western Blotting, Flow Cytometry, for the detection of IL-21. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 3.0 µg of this antibody detected IL-21 in human peripheral blood mononuclear cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human IL-21 sequence near the C-terminus. |
| Epitope | Near C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with pro- and mature IL-21 of both isoform 1 and isoform 2 (IL-21iso). |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Bovine, Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human IL-21 transfected HEK293 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-21 in 10 µg of human IL-21 transfected HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |