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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABF1215 |
| Replaces | 06-548 |
| Description | Anti-SLP76 Antibody |
| Alternate Names |
|
| Background Information | Lymphocyte cytosolic protein 2 (UniProt Q13094; also known as SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76) is encoded by the LCP2 gene (Gene ID 3937) in human. SLP76 is a signaling adaptor molecule expressed in a variety of hematopoietic cells, including lymphocytes, myeloid cells, and platelets. SLP-76 contains three pYESP motifs (Y113 and Y128) that are phosphorylated by ZAP-70 to produce docking sites for SH2-containing proteins, such as exchange factor Vav1, adaptor Nck, resting lymphocyte kinase Rlk, and inducible tyrosine kinase Itk. In addition, SLP-76 binds to the SH3 domain of phospholipase Cγ1 (PLCγ1) and SLP76-deficient T cells show an impaired PLCγ1 activation and calcium mobilization. SLP-76 is essential for mediating TCR signaling, Lcp2-/- mice exhibit impaired thymic development and their T cells fail to develop beyond stage III at the double-negative stage. SLP76 is also a critical determinant of NK-cell development and the the underlying “missing-self” recognition mechanism that prevents NK cells from destroying cells that express self major histocompatibility complex (MHC) class I molecules. SLP76 contains an N-terminal sterile-α motif or SAM (human a.a. 15-81 and moust a.a. 12-78) and a C-terminal SH2 domain (human/mouse a.a. 422-530). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-SLP76, Cat. No. ABF1215 is validated for use in Immunoprecipitation and Western Blotting for the detection of SLP76. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated SLP76 from 500 µg of Jurkat cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a central region sequence within the N-terminal half of human SLP76. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody targets a central region sequence within the N-terminal half of human SLP76. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~76 kDa observed. 60.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SLP76 in 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |