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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, ICC, FC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABF117 |
| Description | Anti-IFIT1/p56 Antibody |
| Alternate Names |
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| Background Information | IFIT1, also known as GARG-16, Glucocorticoid-attenuated response gene 16 protein or Interferon-induced 56 kDa protein, IFI-56K, or P56, and encoded by the gene lfit1/Garg16, Ifi56, Isg56, is an interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2-O-methylation of the 5 cap and bear a 5-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. IFIT1 directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2-O-methylase for their mRNAs or by stealing host cap containing the 2-O-methylation (cap snatching mechanism). IFIT1 is a component of an interferon-dependent multiprotein complex that is at least composed of IFIT1, IFIT2 and IFIT3 and interacts with EIF3F, RPL15, TMEM173 and EEF1A1 and is important in antiviral defense, and innate immune response. IFIT1 is localized to the cytoplasm and expressed by most cells, particularly in the immune system and epithelial cells. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect IFIT1 using this rabbit polyclonal antibody, Anti-IFIT1/p56 Antibody validated for use in western blotting, ICC & Flow Cytometry. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IFIT1/p56 in 10 µg of TF-1 and MOLT-4 cell lysates. Western Blotting Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in HT1080 cell lysates transfected with mouse p56 vector and not in HT1080 cell lysates transfected with empty vector (Terenzi, F., et al. (2007). J Virol. 81(16):8656-8665.). Western Blotting Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated wild type Stat1 MEF cell lysates and not in IFN-beta treated Stat1 knockdown MEF cell lsyates (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053.). Western Blotting Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated wild type p56 MEF cell lysates and not in IFN-Beta treated p56 knockdown MEF cell lysates (Fensterl, V., et al. (2012). PLoS Pathog. 8(5):e1002712.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated MEF cell lysates (Terenzi, F., et al. (2007). J Virol. 81(16):8656-8665.). Flow Cytometry Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in bone marrow cells and splenocyotes upon injection with dsRNA, IFN-alpha, and VSV (Terenzi, F., et al. (2007). J Virol. 81(16):8656-8665.). Flow Cytometry Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated CD3+ mature T cells, and in IFN-Beta treated myeloid Dendritic cells, but not in IFN-Beta treated plasmacytoid Dendritic cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053.). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse IFIT1/p56. |
| Host | Rabbit |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse ovary tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IFIT1/p56 in 10 µg of mouse ovary tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |