| Description | |
|---|---|
| Catalogue Number | ABF1067 |
| Description | Anti-Tapasin |
| Alternate Names |
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| Background Information | Tapasin (UniProt: Q9R233; also known as TPN, TPSN, TAP-associated protein, TAP-binding protein) is encoded by the Tapbp (also known as Tapa) gene (Gene ID: 21356) in the murine species. Tapasin is a single-pass type I membrane glycoprotein protein that is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide. It is synthesized with a signal peptide of 23 amino acids, which is subsequently cleaved to generate mature Tapasin. It assists in loading of antigenic peptides on to class I molecules in the endoplasmic reticulum (ER). It is also reported to support the binding of high-affinity peptides to MHC class I in conjunction with ERp57. Defects in Tapasin expression are linked to destabilization of the MHC class I loading complex and diminution in the expression of MHC molecules at the cell surface. Tapasin is reportedly down-regulated in several human carcinomas and its re-expression in Tapasin-deficient cancer cells restores the expression of MHC class I antigen complexes and augment cancer cell immunogenicity, which helps in longer survival in animals bearing metastatic tumors. (Ref.: Lou, Y et al. (2008). Clin Cancer Res. 14(5); 1494-1501). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tapasin Antibody, Cat. No. ABF1067, is a rabbit polyclonal antibody that detects Tapasin in murine cells and has been tested for use in Immunoprecipitation and Western Blotting. |
| Key Applications |
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| Application Notes | Immunoprecipitation Analysis: A representative lot detected Tapasin in K42 cells (Lum, R., et. al. (2016). J Biol Chem. 291(37):19631-41) and L-cells and BW5147 thymoma cells (Suh, W.K., et. al. (1999). J Immunol. 162(3):1530-40). Western Blotting Analysis: A representative lot detected Tapasin in CMT.64 cells treated with IFN- (Lou, Y., et. al. (2008). Clin Cancer Res. 14(5):1494-501). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine Tapasin. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Tapasin in murine cells. It targets an epitope within 20 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed; 49.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH/3T3 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tapasin in 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
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| Material Size | 100 µL |