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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, ChIP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE70 |
| Description | Anti-BAF180 Antibody |
| Alternate Names |
|
| Background Information | BRG1-associated factor 180 (BAF180) is a subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex, and is known to contain 6 bromo domains, 2 BAH domains, and a single HMG box DNA-binding domain. BAF180 has a critical and active role in the transcriptional activation and repression of specific genes via chromatin remodeling, including genes involved in the development of the heart, thus suggesting a role in mammalian development. Recent studies demonstrate BAF180 to suppress tumorigenesis through its ability to regulate both baseline and signal-dependent p21 transcription by its binding of the p21 promoter. Expression of BAF180 is reported in a wide range of tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-BAF180 Antibody is a Rabbit Polyclonal Antibody for detection of BAF180 also known as protein polybromo-1, BRG1-associated factor 180 & has been validated in WB. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP (Xia, W., et al. (2008). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human BAF180. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). SUBUNIT STRUCTURE: Component of the SWI/SNF-B (PBAF) chromatin-remodeling complex, which contains at least SMARCA4/BRG1, SMARCB1/SNF5/INI1/BAF47, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PB1/BAF180, ARID2/BAF200, ARID1A/BAF250A or ARID1B/BAF250B and actin. Interacts with PHF10/BAF45A (By similarity). SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICTY: Widely expressed. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Contains 2 BAH domains. Contains 6 bromo domains. Contains 1 HMG box DNA-binding domain. SEQUENCE CAUTION: The sequence AAI15010.1 differs from that shown. Reason: Contaminating sequence. The sequence AAI15011.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI15012.1 differs from that shown. Reason: Erroneous termination at position 78. Translated as Arg. The sequence BAB71210.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~193 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected BAF180 in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |