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Millipore Detect Nsd Using This Rabbit Polyclonal Antibody, Anti-Nsd2 Antibody Validated For Use In Western Blotting, Ihc & Ip. - Mill (Additional S&H or Hazmat Fees May Apply)
Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein
WHSC1
Background Information
Nuclear SET domain-containing protein 2 (NSD2) is also called Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein (MMSET), Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1). NSD2 is a histone methyltransferase that acts on histone H3 Lys-27 (H3K27me). Isoform RE-IIBP is thought to be a transcription regulator that uses HADC recruitment to binding DNA and suppress IL5 transcription. Chromosomal aberrations to WHSC1 are linked to multiple myeloma tumors.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect NSD using this rabbit polyclonal antibody, Anti-NSD2 Antibody validated for use in western blotting, IHC & IP.
Key Applications
Western Blotting
Immunohistochemistry
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NSD2 in human cerebellum and thalamus tissues.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NSD2 in human kidney tissues.
Immunoprecipitation Analysis: 5 µg of this antibody immunoprecipitated NSD2 from 500 µg of HCT-116 cell lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to a region near the C-terminus of human NSD2.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody is expected to cross-react with isoforms 1 (152 kDa), 3 (71 kDa), and 4 (66 kDa, REII-BP; IL-5 promoter REII-region-binding protein) based upon immunogen design.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001035889.1
Gene Symbol
WHSC1
MMSET
NSD2
TRX5
Purification Method
Affinity Purfied
UniProt Number
O96028
Molecular Weight
~150 kDa observed. Uniprot describes 7 different isoforms ranging from 30 kDa to 152 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected NSD2 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
