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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, IH(P), ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE364 |
| Description | Anti-Tet2 Antibody |
| Alternate Names |
|
| Background Information | Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tet2 detects levels of Tet2 proteins & has been published & validated for use in WB, IHC & ICC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected Tet2 in rat medulla tissue. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tet2 in human H9 Embryonic Stem cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Tet2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~140 kDa observed. Three isoforms at 223 kDa (isoform 1), 130 kDa (isoform 2), and 133 kDa (isoform 3) are known to exist. This antibody should recognize all three isoforms based on sequence homology. An uncharacterized band may be observed at ~36 kDa in some cell lysates. (Note: Isoforms 2 and 3 may observed at low concentrations, while isoform 1 may be detected at higher concentrations in certain cell lysates.) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse Embryonic Stem cells. Western Blotting Analysis: 1 µg/mL of this antibody detected Tet2 in 10 µg of mouse Embryonic Stem cells (Note: Isoforms 2 and 3 may observed at low concentrations or longer exposure times, while isoform 1 may be detected at higher concentrations or longer exposure times in certain cell lysates.). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |