Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, IH(P), IF | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2875 |
| Description | Anti-Nek6 |
| Alternate Names |
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| Background Information | Serine/threonine-protein kinase Nek6 (UniProt: Q9HC98; also known as EC: 2.7.11.1, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512) is encoded by the NEK6 gene (Gene ID: 10783) in human. Nek6, a member of the NIMA-related serine/threonine kinase family that plays an important role in mitotic cell cycle progression and is required for chromosome segregation at metaphase-anaphase transition. It localizes to spindle microtubules in metaphase and anaphase and to the mid-body during cytokinesis. Nek6 is ubiquitously expressed, with highest expression in heart and skeletal muscle. Its levels are up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers. Nek6 is shown to be directly phosphorylated by Chk1 and Chk2, suggesting that it is a direct target of the DNA damage checkpoint. It is also phosphorylated upon IR or UV-induced DNA damage. Nek6 is activated by phosphorylation at Ser206. Although expression of Nek6 is tightly controlled in normal cells, this protein is highly overexpressed in various human cancers and regulates tumor progression and its expression and activity levels directly correlate to tumorigenicity of human cancer cells. Inhibition of Nek6 can lead to apoptosis. (Ref.: Nassirpour, R et al. (2010). Mol. Cancer Res. 8(5); 717-28). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Nek6, Cat. No. ABE2875, is a highly specific rabbit polyclonal antibody that targets Serine/threonine-protein kinase Nek6 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nek6 in human pancreas and human cervical cancer tissue. Immunofluorescence Analysis: A representative lot detected Nek6 in HeLa cells that were transfected with Nek6 siRNA (ORegan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90). Western Blotting Analysis: A representative lot detected Nek6 in IP blot from lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole, HeLa cells were either mock transfected or transfected (ORegan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; ORegan, L., et. al. (2015). J Cell Biol. 209(3):349-58). Immunoprecipitation Analysis: A representative lot detected Nek6 in lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole (ORegan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; ORegan, L., et. al. (2015). J Cell Biol. 209(3):349-58). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the first 15 amino acids from the N-terminal region of human Serine/threonine-protein kinase Nek6. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Serine/threonine-protein kinase Nek6 in human cells. It targets an epitope with in the first 15 amino acids in the N-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~35 kDa observed; 35.71 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HT1080 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Nek6 in 10 µg of HT1080 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |