Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ICC, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2600-100UL |
| Description | Anti-DNA Helicase B (HELB) |
| Alternate Names |
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| Background Information | DNA helicase B (UniProt: Q6NVF4; also known as EC: 3.6.4.12, HELB) is encoded by the Helb gene (Gene ID: 117599) in murine species. HELB helicase B is is a predominantly nuclear helicase that can undergo phosphorylation at Serine 942 by CDK2 during the G1/S transition that results in its export into the cytoplasm as cells approach and progress through S phase. Its nuclear export signal lies within amino acids 1022-1046. It serves as a 5 -3 DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection. It is shown to bind to RPA1 and this interaction promotes HELB recruitment to chromatin following DNA damage. Following DNA damage it is recruited to sites of double-strand breaks by the Replication Protein A (RPA) complex. Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5-3 ssDNA translocase activity of HELB. HELB is reported to act independently of TP53BP1. Loss of DNA helicase B results in PARP inhibitor resistance in BRCA1-deficient tumor cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH7.4 with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-DNA Helicase B (HELB) Antibody, Cat. No. ABE2600, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected DNA Helicase B (HELB) in Immunocytochemistry applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418). Western Blotting Analysis: A representative lot detected DNA Helicase B (HELB) in Western Blotting applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 227 amino acids from the C-terminal half of murine DNA helicase B. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects DNA helicase B in murine species. It targets an epitope within 227 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 145 kDa observed; 121.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in wild-type mouse embryonic fibroblasts . Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DNA helicase B (HELB) in lysate from wild-type mouse embryonic fibroblasts (MEF) and MEF heterogygotes for HELB. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |