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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1957 |
| Description | Anti-CENP-C Antibody |
| Alternate Names |
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| Background Information | Centromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to cause mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antibody serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CENP-C Antibody, Cat. No. ABE1957, is a highly specific rabbit polyclonal antibody that targets CENP-C and has been tested in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:10,000 dilution from a representative lot immunostained kinetochores in chromosome spreads prepared from interphase DLD-1 human colorectal adenocarcinoma cells hypotonically swollen and fixed with 4% formaldehyde. Immunocytochemistry Analysis: A representative lot was affinity purified and detected a time-dependent loss of kinetochores CENP-C immunoreactivity in 4% formaldehyde-fixed, 0.5% Triton X-100-permeabilized HeLa cells following CENP-C shRNA induction (Falk, S.J., et al. (2015). Science. 348(6235):699-703). Immunocytochemistry Analysis: A representative lot was affinity purified and immunostained kinetochores by indirect fluorescence staining of chromosome spreads prepared from patient-derived, PD-NC4 chromosome variant harboring fibroblasts hypotonically swollen and fixed with 4% formaldehyde (Bassett, E.A., et al. (2010). J. Cell Biol. 190(2):177-185). Western Blotting Analysis: A representative lot was affinity purified and detected a time-dependent CENP-C level in HeLa cells following CENP-C shRNA induction (Falk, S.J., et al. (2015). Science. 348(6235):699-703). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human CENP-C N-terminal fragment. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antiserum targets the N-terminal region of human CENP-C present in both spliced isoforms reported by UniProt (Q03188). Specificity was demonstrated by a reducion of target band detection (Western blotting) and kinetochores staining (chromsome spreads fluoresence staining) following cellular CENP-C shRNA induction (Falk, S.J., et al. (2015). Science. 348(6235):699-703). |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified. |
| UniProt Number |
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| Molecular Weight | ~140 kDa observed. 106.8 kDa (isoform 1) and 61.54 kDa (isoform 2) calculated. The larger-than-calculated target band size is consistent with that reported in the literature (Earnshaw, W.C., et al. (1989). Chromosoma. 98(1):1-12). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in DLD-1 cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected CENP-C in 50,000 cell equivalent of DLD-1 human colorectal adenocarcinoma cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |