Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ChIP-seq, ChIP, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1877 |
| Description | Anti-PAXIP1 |
| Alternate Names |
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| Background Information | PAX-interacting protein 1 (UniProt: Q6NZQ4; also known as PAX transactivation activation domain-interacting protein, PAXIP1) is encoded by the Paxip1 (also known as Ptip) gene (Gene ID: 55982) in murine species. PAXIP1 is a nuclear matrix protein that is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes such as the MLL2/MLL3 complex. In vitro it is shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks. It recruits Tp53bp1 to DNA damage foci and effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by ATM. It also recruits PAGR1A (Pax-Interacting Protein 1-associated glutamate rich protein 1a) to DNA damage sites and PAGR1A:PAXIP1 complex is believed to be essential for cell survival. PAXIP1 expression is reported in all tissues and high expression is observed in embryonic kidney and brain. PAXIP1 contains 6 BRCT domains and BRCT domains 1 and 2 mediate its interaction with PAGR1A and interaction with MLL2/MLL3 is accomplished with BRCT domains 5 and 6. Mice with defective Paxip1 gene are developmentally retarded and usually face embryonic lethality by day 9.5 (E9.5). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PAXIP1, Cat. No. ABE1877, is a highly specific rabbit polyclonal antibody that targets PAX-interacting protein 1 and has been tested for use in ChIP-seq and Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: A representative lot detected PAXIP1 in mouse embryonic fibroblasts (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39). ChIP-seq Analysis: Analysis: A representative lot detected PAXIP1 in ChIP-seq applications (Callen, E., et. al. (2012). Immunity. 37(6):971-85). Immunoprecipitation Analysis: A representative lot detected PAXIP1 in Immunoprecipitation applications (Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93). Immunoprecipitation Analysis: 1 µg from a representative lot detected PAXIP1 in nuclear extract of wild type and PTIP KO mouse embryonic fibroblasts (Courtesy of Dr. Ji-Eun Lee and Dr. Kai Ge at NIH). Western Blotting Analysis: A representative lot detected PAXIP1 in Western Blotting applications (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to 199 amino acids from the N-terminal region of murine PAX-interacting protein 1. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects PAX-interacting protein 1 in human and murine cells. It targets an epitope within 199 amino acids from the N-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed (recombinant); 119.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting with with recombinant Pax-interacting protein 1. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant PAX-interacting protein 1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |