Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1803 |
| Description | Anti-Adenosylhomocysteinase (AHCY) |
| Alternate Names |
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| Background Information | Adenosylhomocysteinase (UniProt: P23526; also known as EC: 3.3.1.1, AdoHcyase, S-adenosyl-L-homocysteine hydrolase) is encoded by the AHCY (also known as SAHH) gene (Gene ID: 191) in human. Adenosylhomocysteinase is a homotetrametic enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine. Hence, it regulates the intracellular S-adenosylhomocysteine concentration, which is thought to be important for transmethylation reactions. Two isoforms of adenosylhomocysteinase have been reported that are produced by alternative splicing. It contains three nucleotide binding regions (aa 157-159; 222-227; and 299-301). Defects in AHCY gene cause hypermethioninemia that is characterized with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Adenosylhomocysteinase (AHCY) Antibody, Cat. No. ABE1803, is a highly specific rabbit polyclonal antibody that targets Adenosylhomocysteinase (AHCY) and has been tested for use in Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected Adenosylhomocysteinase (AHCY) in 200 µg of human placental tissue lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Adenosylhomocysteinase (AHCY) in human kidney and human skin tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminus region of human Adenosylhomocysteinase. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Adenosylhomocysteinase in human. It targets and epitope within 12 amino acids from the C-terminus region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~47 kDa observed; 47.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Adenosylhomocysteinase (AHCY) in 200 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |