Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1800-25UG |
| Description | Anti-La-Related Protein 1 |
| Alternate Names |
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| Background Information | La-related protein 1 (UniProt: Q6PKG0; also known as La ribonucleoprotein domain family member 1) is encoded by the LARP1 (also known as KIAA0731, LARP) gene (Gene ID: 23367) in human. La-related protein 1 is a cytoplasmic RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. It contains 1 HTH La-type RNA-binding domain and it associates with the mRNA 5cap in an mTOR-dependent manner and associates with mRNAs containing a 5 terminal oligopyrimidine (5TOP) motif, which is present in mRNAs encoding for ribosomal proteins and several components of the translation machinery. La-related protein 1 is also shown to associates with actively translating ribosomes via interaction with polyadenylate-binding protein 1 (PABPC1/PABP) and stimulates translation of mRNAs containing a 5TOP, thereby regulating cell growth and proliferation. It is shown to be up-regulated in a number of hepatocellular carcinoma cell lines and liver cancer lesions, as well as in patients with hepatocellular carcinoma with a lower survival rate. (Ref.: Fonseca, BD et al. (2015). J. Biol. Chem. 290(26):15996-6020). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-La-Related Protein 1 Antibody, Cat. No. ABE1800-25UG, is a highly specific rabbit polyclonal antibody, that targets La-related protein 1 and has been tested in Western Blotting and Immunocytochemistry and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected La-Related Protein 1 in HEK293 cells. Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected La-Related Protein 1 in human cerebellum, human tonsil, and human pancreas tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 15 amino acids from the N-termnal half of huamn La-related protein 1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 123 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected La-Related Protein 1 in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |