Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1797-25UG |
| Description | Anti-Transcription Initiation Factor IIA subunit 1 |
| Alternate Names |
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| Background Information | Transcription initiation factor IIA subunit 1 (UniProt: P52655; also known as General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42) is encoded by the GTF2A1 (also known as TF2A1) gene (Gene ID: 2957) in human. TFIIA is one of the several components of the transcription machinery of RNA polymerase II that plays an important role in transcriptional activation. TFIIA in a complex with TATA-box-binding protein (TBP) mediates transcriptional activity. After initiation, some components of the pre-initiation complex, including TFIIA, remain attached and re-initiate a subsequent round of transcription. Binding of TFIIA to TBP helps to stabilize TBP binding to the TATA element. TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Transcription Initiation Factor IIA subunit 1 Antibody, Cat. No. ABE1797-25UG, is a highly specific rabbit polyclonal antibody, that targets Transcription initiation factor IIA subunit 1 and has been tested in Western Blotting and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Transcription initiation factor IIA subunit 1 in 10 µg of RAW264.7 cell lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Transcription initiation factor IIA subunit 1 in human brain and human testis tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Transcription initiation factor IIA subunit 1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Transcription initiation factor IIA subunit 1 in human cells. It targets an epitope within 15 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~55 kDa kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected Transcription initiation factor IIA subunit 1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |