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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, ChIP-seq, ChIP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE154 |
| Description | Anti-Histone H3.1/H3.2 Antibody |
| Alternate Names |
|
| Background Information | Histone H3.1 and H3.2 are closely related histone variants that differ by only one amino acid. Mammalian cells express three major types of noncentromeric histone H3 variants, H3.1, H3.2, and H3.3. H3.1 is only expressed in mammals, while H3.2 is found in all eukaryotes except for yeast. Recent evidence shows that these nearly identical histone proteins differ in their expression profiles and post-translational modifications (PTMs). H3.2 is enriched in modifications associated with transcriptional silencing (di- and tri-methylation of K27), whereas H3.1 is enriched in both K9 dimethylation and K14 acetylation, suggesting that these variants may have separate biological functions. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Histone H3.1/H3.2 Antibody is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting & ChIP-seq. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Histone H3.2 in recombinant Histone H3.2 and mouse Embryonic Stem cell lysates. Chromatin Immunprecipitation: A representative lot detected Histone H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University). Chromatin Immunoprecipitation Sequencing: A representative lot detected Histone H3.1/H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Histone H3.1 and H3.2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody is expected to demonstrate broad species reactivity. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed. Uncharacterized bands may be observed at ~25 kDa and ~50 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Histone H3.1 and H3.2 in 10 μg of HeLa acid extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |