Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1409-25UG |
| Description | Anti-Lysine-Specific Demethylase 4C (JMJD2C) |
| Alternate Names |
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| Background Information | Lysine-specific demethylase 4C (UniProt: Q9H3R0; also known as EC: 1.14.11.-; Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, Jmjd2c) is encoded by the KDM4C (also known as GASC1, JHDM3C, JMJD2C, KIAA0780) gene (Gene ID: 23081) in human. Jmjd2c is an ubiquitously expressed histone demethylase that specifically demethylates Lys-9 and Lys-36 residues of histone H3. It is shown to demethylate only the trimethylated H3 and does not affect mono- and dimethylated residues. It does neither demethylate histone Lys-4 or Lys-27 of histone H3 nor the Lys-20 of histone H4. Human Jmjd2C contains one jumonji N domain (aa 16-58), a catalytic jumonji C domain (aa 144-310), two PHD zinc finger regions (aa 689-865), and two TUDOR methylated-histone binding domains (aa 877-991). Jmjd2c, along with its closely demethylase Jmjd2b, plays a vital role in self-renewal of embryonic stem cells (ESC) and in generation of induced pluripotent stem cells. Full-length Jmjd2b and Jmjd2c are shown to restore the normal ESC growth phenotype, ESC colony morphology, and also SSEA-1 expression. Jmjd2c is reported to assist polycomb repressive complex (PRC) in transcriptional repression. It is overexpressed in several esophageal squamous cell carcinomas. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Lysine-Specific Demethylase 4C (JMJD2C) Antibody, Cat. No. ABE1409-25UG, is a highly specific rabbit polyclonal antibody, that targets Lysine-specific demethylase 4C and has been tested in Western Blotting and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of C2C12 and HEK293 nuclear extract. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in human tonsil, human cervical cancer, and human liver cancer tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-Conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Lysine-specific demethylase 4C. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects Lysine-specific demethylase 4C in multiple human and mouse cell types. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 120 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in BALB/3T3 clone A31 cell nuclear extract. Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of BALB/3T3 clone A31 nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |