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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, PIA, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1397 |
| Description | Anti-trimethyl STAT3 (Lys180) Antibody |
| Alternate Names |
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| Background Information | Signal transducer and activator of transcription 3 (UniProt P40763; also known as Acute-phase response factor, DNA-binding protein APRF) is encoded by the STAT3 (also known as ADMIO, APRF, HIES) gene (Gene ID 6774) in human. STAT proteins are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 is known to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). STAT3 gene knockout in mice is lethal at the early embryonic stage. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-trimethyl STAT3 (Lys180) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay, Immunoprecipitation for the detection of trimethyl STAT3 (Lys180). |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated STAT3 trimethylated on Lys180 in 0.5 mg of mouse embryonic stem cell lysate. Peptide Inhibition Assay: Target specificity of a representative lot was confirmed by immunogen peptide blocking prior to Western blotting analysis (0.5 µg/mL) ) of mouse embryonic stem cell lysate (10 µg/lane) . Only the immunogen peptide, but not the corresponding peptide without the Lys180 trimethylation modification, blocked the detection of the target band. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the STAT3 K180me3 of human trimethyl STAT3 (Lys180) |
| Epitope | STAT3 K180me3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Trimethylation site is present in all three human spliced isoforms. Reactivity toward Stat3 with mono- or dimethylated Lys180 has not been tested. |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Predicted to react with Human, Rat, Bovine, Chicken, Porcine, Orangatan based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~88 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse embryonic stem cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected STAT3 trimethylated on Lys180 in 10 µg of mouse embryonic stem cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |