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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, ChIP, ChIP-seq, DB, ELISA | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1363 |
| Description | Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody |
| Alternate Names |
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| Background Information | H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 0.05% ProClin300. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Dot Blot and ELISA for the detection of acetyl Histone H2A.Z (Lys 5, 7, 11). |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from HeLa cells. Dot Blot Analysis: 0.05 ug/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in H2A.Z peptides. Chromatin Immunoprecipitation (Seq.) Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from K562 cells. Immunocytochemistry Analysis: A representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human acetyl Histone H2A.Z (Lys 5, 7, 11) near the N-terminus. |
| Epitope | Near N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Human, mouse, wide range expected. |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~16 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in 10 µg of sodium butyrate treated HeLa acid extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |