Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1359-25UL |
| Description | Anti-PHD finger protein 14 (PHF14) |
| Alternate Names |
|
| Background Information | PHD finger protein 14 (UniProt: O94880) is encoded by the PHF14 (also known as KIAA0783) gene (Gene ID: 9678) in human. PHF14 is a member of the PHD finger protein family that serves as a chromatin-binding protein. It contains four putative PHD fingers, two coiled-coil regions, and four zinc fingers. PHF14 is highly conserved from C. elegans to humans and is considered as a transcriptional regulator of target gene expression. PHF14 has been identified as a regulator of mesenchyme growth in embryonic tissues. Two isoforms of PHF14 have been described that are produced by alternative splicing. PHF14 interacts with histones via its PHD1 and PHD3 domains indicating its role in epigenetic regulation. It is involved in chromatin-mediated transcriptional regulation and chromatin dynamics. During cell division, PHF14 is shown to be directly bound to and co-localized with KIF4A to form a functional complex. PHF14 is shown to be overexpressed in lung cancer and this overexpression is associated with poor prognosis of non-small cell lung carcinoma. (Ref.: Zhang, L., et al. (2017). Oncotarget. 8(12); 19684-19698; Yang, B., et al. (2017). Sci Rep. 7; 39888). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PHD finger protein 14 (PHF14) Antibody, Cat. No. ABE1359, is a highly specific rabbit polyclonal antibody that detects PHD finger protein 14 and has been tested for use in Immunocytochemistry and in Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PHD finger protein 14 (PHF14) in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human PHD finger protein 14. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects PHD Finger Protein 14 in human cells. It targets an epitope within 16 amino acids from the N-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse and Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Molecular Weight | 100.0 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected PHD finger protein 14 (PHF14) in HEK293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |