Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M | WB, ICC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1327 |
| Description | Anti-DPF2 |
| Alternate Names |
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| Background Information | Zinc finger protein ubi-d4 (UniProt: Q92785; also known as Apoptosis response zinc finger protein, BRG1-associated factor 45D, BAF45D, D4, zinc and double PHD fingers family 2, Protein requiem, DFP2) is encoded by the DPF2 (also known as BAF45D, REQ, UBID4) gene (Gene ID: 5977) in human. BAF45D is a member of the requiem/DPF protein family and is ubiquitously expressed. It may serve as a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. It is also believed to have a role in the development and maturation of lymphoid cells. It contains 3 zinc-finger domains (C2H2-type aa 209-232; PHD type I aa 270-330; and PHD type 2 aa 327-377). Two isoforms of BAF45D have been described that are generated via alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DPF2, Cat. No. ABE1327, is a highly specific rabbit polyclonal antibody that targets BRG1-associated factor 45D and has been tested in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected DPF2 in 10 µg of PC12 cell lysate. Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected DPF2 in A431 and HepG2 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated liner peptide that corresponding to 17 amino acids from the N-terminal region of human BRG1-associated factor 45D (DPF2). |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects BRG1-associated factor 45D in human, mouse, and rat species. It targets an epitope within 17 amino acids from the N-terminal half. The immunogen sequence is conserved in both isoforms. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~44 kDa observed; 44.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH3T3 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected DPF2 in 10 µg of NIH3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |