Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1323 |
| Description | Anti-Forkhead box protein K2 (FOXK2) |
| Alternate Names |
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| Background Information | Forkhead box protein K2 (UniProt: Q01167; also known as Cellular transcription factor ILF-1, FOXK1, Interleukin enhancer-binding factor 1) is encoded by the FOXK2 (also known as ILF, ILF1) gene (Gene ID: 3607) in human. Forkhead box protein K2 is a transcriptional regulator that recognizes the core sequence 5-TAAACA-3. It is expressed in both lymphoid and non-lymphoid cells. Three isoforms of Forkhead box protein K2 have been reported that are produced by alternative splicing. It binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Forkhead box protein K2 is reported to positively regulate WNT/beta-catenin signaling by translocating DVL proteins into the nucleus. Higher levels of FOXK1 and FOXK2 are reported in human colorectal cancers and correlate with DVL nuclear localization. Both FOXK1 and FOXK2 proteins are shown to act as transcriptional repressors of autophagy in muscle cells and fibroblasts. They specifically recruit Sin3A HDAC complexes to restrict acetylation of histone H4 and expression of critical autophagy genes. The loss of FOXK1 and FOXK2 proteins results in the upregulation of several genes controlling the initiation and nucleation of autophagosome formation. (Ref.: Bowman, CJ., et al. (2014). Nat. Cell Biol. 16(12); 1202-1214). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Forkhead box protein K2 (FOXK2), Cat. No. ABE1323, is a highly specific rabbit polyclonal antibody that targets Forkhead box protein K2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected Forkhead box protein K2 (FOXK2) in HeLa cell lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Forkhead box protein K2 (FOXK2) in human cerebral cortex, human prostate cancer, and human pancreatic cancer tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human Forkhead box protein K2. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Forkhead box protein K2 in human cells. It targets an epitope within 17 amino acids from the C-terminal half. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~69 kDa observed; 69.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Forkhead box protein K2 (FOXK2) in HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |