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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Eq, B, Ca | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE12 |
| Description | Anti-phospho CARM1 (Ser228) Antibody |
| Alternate Names |
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| Background Information | CARM1 (coactivator-associated arginine methyltransferase 1) is an arginine N-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues. This protein-arginine methyltransferase that works in conjunction with p300 and GRIP1 to enhance transcription. CARM1 methylation targets include histone H3 at Arg17 and p300 at Arg2142. CARM1 is recruited to promoters upon gene activation together with histone acetyltransferases from the p300 and p160 families. During monocyte inflammatory stimulation, CARM1 acts together with p300 as a coactivator for NF-kB. It is responsible for moving cells toward the inner cell mass in developing blastocysts. CARM1 also plays an important role in androgen receptors and may play a role in prostate cancer progression. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho CARM1 (Ser228) Antibody is a Rabbit Polyclonal Antibody for detection of phospho CARM1 (Ser228) also known as Protein arginine N-methyltransferase 4 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human CARM1 phosphorylated at Ser228. |
| Epitope | Phosphorylated Ser228 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes CARM1 phosphorylated at Ser228. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Mouse, Rat, Equine, Bovine, and Canine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM]. |
| Gene Symbol |
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| Modifications |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg-17 and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at Arg-2142, which may loosen its interaction with NCOA2/GRIP1, and at Arg-580 and Arg-604 in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone]. SUBUNIT STRUCTURE: Homodimer Probable. Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, CREBBP and CTNNB1. Interacts with SNRPC (By similarity). Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1. SUBCELLULAR LOCATION: Nucleus. Cytoplasm TISSUE SPECIFICTY: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. MISCELLANEOUS: Methylation of H3-R17 by CARM1 is stimulated by preacetylation of H3-K18/H3-K23 by EP300 and blocked by citrullination of H3-R17 by PADI4 (By similarity). SEQUENCE SIMILARITIES: Belongs to the protein arginine N-methyltransferase family. |
| Molecular Weight | ~63 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in EGF treated and untreated A431 cell lysates. Western Blot Analysis: 2 µg/mL of this antibody detected CARM1 on 10 µg of EGF treated and untreated A431 cell lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |