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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABD116 |
| Description | Anti-Human Oct-4 Antibody |
| Alternate Names |
|
| Background Information | Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Human Oct-4 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Human Oct-4. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected human Oct-4 in 10 µg of H9 human ES cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-EDC-conjugated linear peptide corresponding to the unknown of Human Oct-4. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells). |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Chimpanzee, Goat based on 100% sequence homology. The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Antigen Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~40 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in H9 human ES cell lysate and mouse ES cells lysate. Western Blotting Analysis: A 0.25 µg/mL dilution of this antibody detected human Oct-4 in 10 µg of H9 human ES cell lysate but not in 10 µg of mouse ES cells lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |