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Millipore This Anti-Gadd153/Ddit3/Chop Antibody Is Validated For Use In Western Blotting For The Detection Of Gadd153/Ddit3/Chop. - Mill (Additional S&H or Hazmat Fees May Apply)
Growth arrest and DNA damage-inducible protein GADD153
GADD153/DDIT3/CHOP
Background Information
DNA damage-inducible transcript 3 protein (UniProt P35638; also known as CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153) is encoded by the DDIT3 (also known as CEBPZ, CHOP, CHOP10, GADD153) gene in human (Gene ID 1649). CHOP is a proapoptotic unfold protein response (UPR) gene and a well established marker for ER stress and UPR activation. The accumulation of unfolded or misfolded proteins in the ER induces the activation of three main UPR pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CHOP upregulation.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-GADD153/DDIT3/CHOP antibody is validated for use in Western Blotting for the detection of GADD153/DDIT3/CHOP.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human GADD153/DDIT3/CHOP near the N-terminus.
Epitope
Near N-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Bovine, Mouse, Rat, Monkey, Porcine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_004074
Gene Symbol
DDIT3
CHOP
CHOP10
GADD153
Purification Method
Affinity Purfied
UniProt Number
P35638
Molecular Weight
~29 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Tunicamycin treated HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD153/DDIT3/CHOP in 10 µg of Tunicamycin treated HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
