Name: Millipore Anti-Aatf Antibody/Rb-Binding Protein Che-1
SKU: MILL-ABC953
Price: 511.84 USD

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC, IP, ChIP	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	ABC953
Description	Anti-AATF Antibody/Rb-binding protein Che-1
Alternate Names	Protein AATF Apoptosis-antagonizing transcription factor Rb-binding protein Che-1
Background Information	AATF/Rb-binding protein Che-1, also known as Protein Apoptosis-antagonizing transcription factor (Protein AATF), Rb-binding protein Che-1, and encoded by the gene name AATF/ CHE1/ DED/ HSPC277, is a member of the AATF family. Recent studies have indicated that AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binds to the pocket region of RB1, and consequently it may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. In addition, AATF antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. AATF/Rb-binding protein Che-1 is expressed at high levels in brain, heart, kidney, placenta and thymus.

Product Information
Format	Serum
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-AATF antibody is validated for use in WB, IC, IP, ChIP for the detection of AATF.
Key Applications	Western Blotting Immunocytochemistry Immunoprecipitation Chromatin Immunoprecipitation (ChIP)
Application Notes	Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in U2OS, T98G, LoVo, HT29, MCF7, HeLa, LIPARI, Jurkat, and U2OS cells were immunoprecipitated with anti-Rb (Fanciulli, M., et al. (2000). FASEB. 14:904-912). Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357). Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in NIH 3T3 transfected cells, Synchronized NIH 3T3, HFF human fibroblasts, and HEK293 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399). Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, AT fibroblasts, NIH 3T3 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486). Immunocytochemistry Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357). Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357). Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399). Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399). Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486). Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in Synchronized NIH 3T3, HFF human fibroblasts, HEK293, Rb transfected HEK293 cells, and Che-1 transfected NIH3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399). Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).

Biological Information
Immunogen	Linear peptide corresponding to the POLR2J domain of human AATF/Rb-binding protein Che-1.
Epitope	POLR2J domain
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_036270
Gene Symbol	AATF CHE1 DED
Purification Method	Unpurified
UniProt Number	Q9NY61
Molecular Weight	~70 kDa observed. The calculated molecular weight is 63 kDa, however AATF/Rb-binding protein Che-1 has been shown as a ~70 kDa band in western blots (Guo, G., et al. (2004). J. Biol. Chem. 279:4596-4603).

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected AATF/Rb-binding protein Che-1 in 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Specifications

More Information
SKU	MILL-ABC953
Featured	No
Supplier Part Number	ABC953
UM	EA
UNSPSC	12352203
Manufacturer Name	Millipore
ProductLine	MILL
Qty	1
MinOrderQty	1
Weight	10.000000
Lead Time	10
Hazardous	N
Energy Star	No
Green	No
ACT Ecolabel	No
Controlled	N

NETA PART NO.	Quantity

Millipore Anti-Aatf Antibody/Rb-Binding Protein Che-1

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Key Specifications Table

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