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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC926 |
| Description | Anti-TRIM11 Antibody |
| Alternate Names |
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| Background Information | E3 ubiquitin-protein ligase TRIM11 (UniProt Q96F44; also known as Protein BIA1, RING finger protein 92, Tripartite motif-containing 11, Tripartite motif-containing protein 11) is encoded by the TRIM11 (also known as BIA1, RNF92) gene (Gene ID 81559) in human. TRIM11 belongs to a family of more than 70 TRIM/RBCC proteins characterized by their shared tripartite motif structure, also known as the RBCC (RING finger, B-box, and coiled-coil) domain. The B-box and coiled coil regions are believed to participate in protein-protein interactions and formation of macromolecular complexes. TRIM proteins are involved in diverse cellular processes, including cell proliferation, differentiation, oncogenesis, and apoptosis. The presence of a RING domain suggests that TRIM proteins function as E3 ubiquitin ligases. The neuroprotective peptide humanin, activator-recruited cofactor (ARC) 105-kDa component (ARC105), the transcription factors Pax6 and PHOX2B are all known subrates targeted by TRIM11-mediated degradation through the ubiquitin-proteasome system. In addition, TRIM11 is also reported to interfere with the early steps of HIV-1 replication in host cells in a RING domain-dependent manner. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect TRIM11 using this rabbit polyclonal Anti-TRIM11 Antibody, Cat. No. ABC926, validated for use in Western Blotting and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected TRIM11 in human prostate cancer, human colon cancer, human melanoma, and human kidney tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human TRIM11. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody is expected to react with all three spliced isoforms of human TRIM11. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rhesus Macaque, Canine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~53 kDa observed. 52.8 kDa (isoform 1), 43.0 kDa (isoform 2), and 52.6 kDa (isoform 3) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in murine macrophage RAW264.7 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected TRIM11 in 10 µg of murine macrophage RAW264.7 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |