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Millipore This Anti-Menin/Men1 Antibody Is Validated For Use In Western Blotting For The Detection Of Menin/Men1 . - Mill (Additional S&H or Hazmat Fees May Apply)
Menin (UniProt O00255) is a nuclear protein encoded by the MEN1 gene (also known as MEAI, SCG2) (Gene ID 4221) in human. MEN1 is a tumor suppressor gene frequently mutated in the familial multiple endocrine neoplasia type I (MEN-1) cancer syndrome characterized by tumors in multiple endocrine organs including the parathyroid, pancreatic islets, and the pituitary. Menin mediates cell proliferation and differentiation actions of transforming growth factor-beta (TGF-beta) ligand family members. Menin is reported to associate with histone methyltransferases (HMT) MLL1 (KMT2A) and MLL4 (KMT2B) to form menin-HMT complexes in both human and murine model systems. In addition, menin is also shown to be involved in DNA repair with FANCD2.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Menin/MEN1 Antibody is validated for use in Western Blotting for the detection of Menin/MEN1 .
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected endogenous Menin in TGP61 nuclear extract, as well as exogenously expressed Menin in transfected NIH3T3 cells (Jin, S., et al. (2003). Cancer Res. 63(14):4204-4210).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human Menin/MEN1.
Epitope
C-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Expected to react with all three spliced variants: Isoform 1 (Long), Isoform 2 (Short), and Isoform 3.
Species Reactivity
Mouse
Species Reactivity Note
Mouse. Predicted to react with Human, Bovine, Canine, Rat based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_000235
Gene Symbol
MEN1
SCG2
Purification Method
Unpurified
UniProt Number
O00255
Molecular Weight
~70 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Tamoxifen treated MEF 545T and MEF 545D cell lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Menin/MEN1 in 10 µg of Tamoxifen treated MEF 545T and MEF 545D cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
