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Millipore Anti-Gli1 Antibody Is A Highly Specific Rabbit Polyclonal Antibody, That Targets Gli1 & Has Been Tested In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Zinc finger protein GLI1 is also called Glioma-associated oncogene and Oncogene GLI. GLI1 is a transcriptional activator that regulates specific genes during normal development. GLI1 also mediates SHH signaling (and therefore cell proliferation and differentiation) and possibly is involved in craniofacial, central nervous system, gastrointestinal tract, and digital development. GLI1 is expressed in the testis, myometrium, fallopian tube, and brain.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-GLI1 Antibody is a highly specific rabbit polyclonal antibody, that targets Gli1 & has been tested in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: A 1:500 dilution from a representative lot detected GL1 in A431, HEK293, HEPG2, and PC3 cells.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GLI1 in human cerebellum and thalamus tissues.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GLI1 in rat testis tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human GLI1.
Host
Rabbit
Species Reactivity
Human
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_005260
Gene Symbol
GLI1
GLI
Purification Method
Affinity Purfied
UniProt Number
P08151
Molecular Weight
~150 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected GLI1 in 10 µg of A549 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
