Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC1451-25UG |
| Description | Anti-PDGF-D |
| Alternate Names |
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| Background Information | Platelet-derived growth factor D (UniProt: Q9GZP0; also known as PDGF-D, Iris-expressed growth factor, Spinal cord-derived growth factor B, SCDGF-B) is encoded by the PDGFD (also known as IEGF, SCDGFB, MSTP036, UNQ1899/PRO4345) gene (Gene ID: 80310) in human. Four PDGF ligands known as PDGF-A, PDGF-B, PDGF-C, and PDGF-D have been described that exert their effect by binding to their cognate receptors. PDGF-D is expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen, and colon. PDGF-D is synthesized with a signal peptide (aa 1-18), which is subsequently removed. The latent form of PDGF-D contains a CUB domain at its N-terminal region, a growth factor domain and a hinge region in between. The latent form is activated by the proteolytic action of matriptase that removes the CUB domain, which results in unmasking of the receptor-binding epitopes on the core domain. YR247GR249S within the PDGF-D hinge region is shown to be the cleavage site for removal of the inhibitory CUB domain. PDGF-D plays an important role in wound healing and induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. PDGF-D also induces cellular transformation and promotes tumor growth by accelerating the proliferation rate of tumor cells and by stimulation of tumor neovascularization. (Ref.: Huang, W., and Kim, H-R, C (2015). J. Biol. Chem. 290(14); 9162-9170). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS (pH 7.4) with 0.09% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PDGF-D, Cat. No. ABC1451, is a rabbit polyclonal antibody that detects Platelet-derived growth factor D in human cells and has been tested for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected PDGF-D in human recombinant-PDGF D (Huang, W., et. al. (2015). J Biol Chem. 290(14):9162-70) and LNCaP cells expressing PDGF-D, BS-C-1 cells transfected with the plasmid pTF7-PDGF D:HIS, recombinant PDGF-D (Ustach, C.V., et. al. (2004). Cancer Res. 64(5):1722-9). Western Blotting Analysis: A representative lot detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 18 amino acids from the C-terminal half of human Platelet-derived growth factor D. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Platelet-derived growth factor D in human cells. It targets an epitope within the growth factor domain from the C-terminal half. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Rabbit based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~90 kDa Full Length PDGF D dimer, ~52 kDa PDGF D hemi-dimer,~32 kDa growth factor dimer in the non-reducing conditions; 42.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting condition media collected from LNCaP cells transfected with PDGF-D expression vector. Western Blotting Analysis: 2 µg/mL of this antibody detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |